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Aseprite

  • You can customize keyboard shortcuts from Edit → Keyboard Shortcuts…
  • Most common hotkeys:
    • Color picker: I
    • Pencil: B
    • Paint bucket: G
    • Rectangular select: M

It’s free to build from source and use Aseprite for commercial projects (reference).

macOS instructions:

brew install cmake ninja
git clone --recursive https://github.com/aseprite/aseprite.git
cd aseprite
./build.sh